FastQCFastQC Report
Thu 2 Jun 2016
DRR024901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109409
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137440.44201325718167017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113290.36434576474178854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74560.23978833276677336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74300.054.293141
GTATCAA137150.043.228061
ATCAACG179800.032.8923953
TCAACGC181650.032.579624
TATCAAC183050.032.3115232
GTGGTAT30850.030.6328491
CAACGCA192550.030.6147445
AACGCAG199050.029.6849126
TGGTATC34800.026.8856372
ACGCAGA219100.026.5607877
CGCAGAG221850.026.0828048
GTACATG162700.024.6205921
GCAGAGT232800.024.2494419
TACATGG163050.023.7603532
TACCTGG26200.022.7902412
ACATGGG166150.022.3244743
GAGTACT138600.021.07430512-13
AGAGTAC219450.020.8303110-11
AGTACTT141550.020.75130812-13
TACAACG6150.020.6412092
Produced by FastQC (version 0.11.5)