FastQCFastQC Report
Thu 2 Jun 2016
DRR024901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109409
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154800.49784380247178806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131000.4213019258643685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93340.30018566229145155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35340.1136550386263113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.052.330721
GTATCAA147300.041.9901471
ATCAACG200250.030.6364733
TCAACGC202200.030.3603574
TATCAAC203250.030.3230512
CAACGCA211300.029.0078815
AACGCAG217050.028.2177586
GTGGTAT34850.028.2077141
TACCTGG26400.025.642442
ACGCAGA245450.024.6807277
CGCAGAG248100.024.3223938
GTACATG162200.023.6046711
TGGTATC40850.023.361722
TACATGG163800.023.1324632
GCAGAGT257800.022.7874229
AGTACTT163000.021.49401912-13
ACATGGG168750.021.1167073
AGAGTAC246100.020.78633510-11
GTACCTG33500.020.7797971
GAGTACT162650.019.93666812-13
Produced by FastQC (version 0.11.5)