FastQCFastQC Report
Thu 2 Jun 2016
DRR024900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698411
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114620.24395481791609974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98870.21043284633890055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58370.12423349085467406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92900.046.9139371
GTATCAA167850.039.382371
ATCAACG203650.031.9265883
TCAACGC206350.031.4830444
TATCAAC215250.030.3602182
CAACGCA217650.029.8259495
AACGCAG227800.028.5573926
GTACATG185650.027.426561
TACATGG181900.026.2082792
ACGCAGA253250.025.2041827
CGCAGAG254000.024.9071718
ACATGGG187550.024.5653953
GTGGTAT47550.023.9277971
GCAGAGT269600.022.8034699
TGGTATC49850.022.634942
TACCTGG35050.020.7910732
AGAGTAC241700.020.44775810-11
GAGTACT155850.020.09898212-13
CATGGGG102300.019.8049964
CAGAGTA263400.019.22713510-11
Produced by FastQC (version 0.11.5)