FastQCFastQC Report
Thu 2 Jun 2016
DRR024900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698411
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112030.23844231592340476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97480.207474399323516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.13555646792075024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99150.046.91531
GTATCAA169350.037.0216521
ATCAACG210800.029.3693853
TCAACGC213250.028.9854094
CAACGCA221950.027.8704155
TATCAAC223600.027.8142762
AACGCAG232600.026.6751526
GTACATG191900.024.656641
TACATGG191600.023.4798932
ACGCAGA262800.023.4092587
CGCAGAG264200.023.1429048
GTGGTAT50850.022.9388481
ACATGGG198550.022.1134593
GCAGAGT284650.020.951929
TGGTATC54350.020.3258172
GAGTACT159850.019.87501112-13
AGAGTAC253900.019.69483410-11
AGTACTT166650.018.8384212-13
GTACTTT169300.018.6129514-15
CATGGGG109650.018.4332754
Produced by FastQC (version 0.11.5)