FastQCFastQC Report
Thu 2 Jun 2016
DRR024899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3211693
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89330.2781399093873543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73860.2299721673273255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.1345396337694792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58050.049.4104731
GTATCAA103200.039.5030631
ATCAACG123700.032.2322433
TCAACGC124350.032.095574
CAACGCA128850.031.0096875
TATCAAC130750.030.7479252
AACGCAG135800.029.6285116
ACGCAGA149450.026.6380867
GTACATG91600.026.539121
CGCAGAG149850.026.283878
TACCTGG26550.025.679992
GTACCTG28450.025.1219121
TACATGG91000.025.0089442
GTGGTAT30300.024.363991
TGGTATC29000.024.321182
GCAGAGT159300.024.134579
ACATGGG94200.023.5090123
TCTGTCG8050.022.7704038
GTACCGG3500.021.4952931
TATAACG3351.8189894E-1221.0541572
Produced by FastQC (version 0.11.5)