FastQCFastQC Report
Thu 2 Jun 2016
DRR024899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3211693
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101060.314662702817486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89550.2788249063655835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56140.1747987743535886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63350.047.9763761
GTATCAA111150.039.1961171
ATCAACG131350.032.8622323
TCAACGC132500.032.5334134
CAACGCA139150.031.0124235
TATCAAC141500.030.5714342
AACGCAG144700.029.9853676
GTACATG91800.026.5990561
ACGCAGA166800.025.8389667
CGCAGAG166200.025.6486538
TACATGG90200.025.5431212
ACATGGG92450.024.1585663
GCAGAGT177350.023.612119
TCGCCGA1154.109097E-922.47706616-17
TACCTGG28350.022.390572
GTACCGG2555.529728E-1022.1402551
GTGGTAT31450.021.541871
GTACCTG29400.021.4436651
TATAACG2659.0221874E-1021.2921642
GAGTACT126700.021.03170212-13
Produced by FastQC (version 0.11.5)