FastQCFastQC Report
Thu 2 Jun 2016
DRR024898_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024898_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354368
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92900.27695232007937115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79960.2383757536442036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48650.1450347725711669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.051.00591
GTATCAA113050.039.670291
ATCAACG138300.031.8137873
TCAACGC139950.031.4718254
TATCAAC144150.030.6223532
CAACGCA146650.030.0001285
TACCTGG31400.029.9424932
AACGCAG153000.028.9683486
GTGGTAT29650.027.270391
ACAACGC6450.026.2364163
GTACCTG37150.025.9407561
ACGCAGA171750.025.5869377
CGCAGAG171900.025.3181578
TAACGCA3350.025.2570674
TGGTATC31250.024.821132
TATAACG4050.024.3754082
GTACATG96050.024.0309931
TCTGTCG7650.023.9607338
GCAGAGT181800.023.4740989
GTACCGG2853.6379788E-1223.0924911
Produced by FastQC (version 0.11.5)