FastQCFastQC Report
Thu 2 Jun 2016
DRR024898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354368
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105150.31347186712966496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91430.27256997443333586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62210.18545967526520643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64300.050.9147951
GTATCAA118200.039.7153851
TACCTGG33150.031.4838942
ATCAACG148450.031.3209633
TCAACGC149700.030.9592174
TATCAAC152800.030.6139072
CAACGCA156050.029.910275
ACAACGC6000.028.991423
AACGCAG162600.028.8499376
GTACCTG37850.028.210171
GTGGTAT32150.025.8963181
ACGCAGA183600.025.4450457
CGCAGAG183200.025.321028
TGGTATC32200.024.8205872
GCAGAGT195050.023.2043749
GTACATG95350.022.9391841
ACCTGGG45000.022.5662963
TATAACG3550.022.513322
TACAACG7850.022.1590482
TACATGG95650.021.479072
Produced by FastQC (version 0.11.5)