FastQCFastQC Report
Thu 2 Jun 2016
DRR024897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461262
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99470.40414226522816343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84390.34287288391077425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58130.23617965092704477No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.10088320544501155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.057.8683131
TACCTGG28000.045.997522
TATAACG2800.045.3260232
GTATCAA92800.042.271851
GTACCTG32550.040.750351
ACAACGC4500.036.559263
ACCTGGG34850.035.877383
GTGGTAT20150.033.3806041
GTATAAC6250.033.113561
ATCAACG119600.032.4238553
TCAACGC121750.031.887944
TGGTATC20800.031.637822
CAACGCA125600.031.1350175
TATCAAC128150.030.4439742
AACGCAG132900.029.5309166
TAACGCA4850.028.1042274
GTACCGG2600.027.1363281
ACGCAGA149500.025.997747
GTACATG86000.025.9793551
CGCAGAG149950.025.8570378
Produced by FastQC (version 0.11.5)