FastQCFastQC Report
Thu 2 Jun 2016
DRR024896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209300
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78490.24457046708004862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63080.19655376561867072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41970.13077618172187083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.053.343671
TACCTGG36550.042.9637072
GTATCAA92700.042.299551
TATAACG4100.040.135332
GTACCTG41250.038.9808731
ATAACGC4650.034.374373
ATCAACG111700.034.1752473
TCAACGC112850.033.8675844
TAACGCA5100.033.183914
TATCAAC117750.032.78122
ACAACGC7050.032.6750143
CAACGCA118550.032.3963055
AACGCAG125000.030.834596
GTGGTAT22250.030.4285961
TCTGTCG11450.030.3776258
TGGTATC23250.028.7149472
GTATAAC10200.027.1957651
ACGCAGA141750.026.759927
ACCTGGG56850.026.6277333
CGCAGAG142500.026.519718
Produced by FastQC (version 0.11.5)