FastQCFastQC Report
Thu 2 Jun 2016
DRR024896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209300
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89670.27940672420777113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.23229364658959895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.1689153397937245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54700.053.7390671
GTATCAA98400.039.7672581
TACCTGG33250.037.607182
TATAACG3950.036.893122
ACAACGC6450.034.983423
GTACCTG38450.033.2713171
ATAACGC4650.031.339323
ATCAACG126450.030.670153
TCAACGC127350.030.4846134
TATCAAC132250.029.431712
CAACGCA133250.029.3111845
TAACGCA4800.028.3959474
TACAACG7800.028.3259222
AACGCAG140850.028.0966686
GTGGTAT26250.027.771531
TGGTATC25700.027.4371452
GTCTCGC10000.027.2788091
GTACCGG3500.026.8756731
ACGCAGA158050.024.889167
CGCAGAG158650.024.7357858
Produced by FastQC (version 0.11.5)