FastQCFastQC Report
Thu 2 Jun 2016
DRR024895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346354
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210480.6289830663462382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167890.5017102195404312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114020.3407290442075166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66880.19985930956497727No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.1565883346472011No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44940.13429541524895452No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34280.1024398494600392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124300.048.5306661
TACCTGG36600.044.0629732
GTATCAA212150.036.9669651
ACCTGGG44000.035.4742853
TACAACG8150.034.0373042
GTACCTG49350.033.5363851
TATAACG4350.032.4259532
ATCAACG264800.029.4370883
TCAACGC268250.029.0922414
TAACGCA5500.029.0619684
TATCAAC273300.028.4892772
CAACGCA279950.027.9607685
AACGCAG290300.027.0926346
ACAACGC10400.026.671473
GTACATG211450.026.0603751
ATAACGC6350.025.1729153
TACATGG210050.025.1372552
GTACAAC13150.024.6708491
ACGCAGA322800.024.2192697
CGCAGAG323000.023.9997928
Produced by FastQC (version 0.11.5)