FastQCFastQC Report
Thu 2 Jun 2016
DRR024894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148580.38236085827119776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125190.32216823157202346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.20605487899969582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77800.055.5903321
GTATCAA147750.043.239731
ATCAACG185050.033.937553
TCAACGC187450.033.552324
TATCAAC190800.033.0893822
TACCTGG35050.032.5918732
CAACGCA196600.032.087225
GTGGTAT31350.031.9398671
AACGCAG205650.030.8564386
TGGTATC32700.029.9024412
ACGCAGA231150.027.1463417
CGCAGAG233200.026.7054628
GTACATG157900.025.871921
GTACCTG46000.025.7533511
TACATGG155600.025.2573452
ACCTGGG43900.025.0561543
GCAGAGT248000.024.334719
GTACCGG5100.023.9659231
TATAACG4200.023.5050412
ACATGGG163150.023.366673
Produced by FastQC (version 0.11.5)