FastQCFastQC Report
Thu 2 Jun 2016
DRR024894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885858
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166110.4274731603676717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145700.3749493676814747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98830.2543325052021973No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.10383807128309887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83900.055.8991551
GTATCAA153500.042.3517841
GTGGTAT33800.032.705681
ATCAACG205050.031.636983
TCAACGC207550.031.2749274
TACCTGG34600.030.9766482
TATCAAC209650.030.7858662
CAACGCA216250.030.1905845
TATAACG4000.029.3802642
TGGTATC36450.029.2754782
AACGCAG224550.029.1580076
ACGCAGA256200.025.4432417
CGCAGAG258850.025.0738248
GTACCTG43450.025.008881
GTACATG155050.024.089111
TACATGG153600.023.4123972
GCAGAGT274950.023.0757149
ACCTGGG45200.022.984213
ACAACGC8100.022.0533353
ACATGGG158800.021.7872813
Produced by FastQC (version 0.11.5)