FastQCFastQC Report
Thu 2 Jun 2016
DRR024893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671548
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120760.2585010364872629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103700.22198209244558764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.131433948661129No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82200.052.218411
GTATCAA146750.039.9496541
ATCAACG181250.032.030413
TCAACGC183850.031.5495034
GTGGTAT37250.030.7955681
CAACGCA191700.030.282095
TATCAAC192650.030.1385692
AACGCAG199750.029.1784326
TGGTATC37100.028.2589242
ACGCAGA222250.025.8649547
CGCAGAG222300.025.4777288
GTACATG137650.024.6937331
TACCTGG36800.023.8901272
TACATGG134000.023.857712
GCAGAGT238350.023.209969
ACATGGG137700.022.3946083
TCTGTCG11450.021.7562228
GTACCTG43050.021.6230371
TATAACG4400.021.3698752
AGAGTAC222700.020.15562410-11
Produced by FastQC (version 0.11.5)