FastQCFastQC Report
Thu 2 Jun 2016
DRR024893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671548
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137590.294527638375973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121710.2605346236408146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78560.16816695450844132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87350.053.7925951
GTATCAA158400.039.6114351
ATCAACG203250.030.527993
TCAACGC206450.030.0730634
TATCAAC210150.029.6598512
CAACGCA214500.028.9006275
AACGCAG223900.027.8762116
ACGCAGA253600.024.4055777
CGCAGAG252800.024.371278
GTGGTAT41150.024.2318151
GTACATG138450.024.1883891
TACCTGG38150.023.7803152
TACATGG136600.023.5030822
GCAGAGT270900.022.4133139
TGGTATC41300.022.3080122
ACATGGG140350.021.803363
ACAACGC8900.021.6545263
GTACCTG42600.021.5300451
TATAACG5100.021.198862
TAACGCA5900.020.7114774
Produced by FastQC (version 0.11.5)