FastQCFastQC Report
Thu 2 Jun 2016
DRR024892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311924
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91810.2772104673899522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74400.22464283600710644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48170.14544415874277308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.052.826391
GTATCAA111900.040.460411
TACCTGG32050.035.4988942
ATCAACG138500.032.2124863
TCAACGC138950.032.1062244
GTACCTG36750.031.7269551
TATCAAC142500.031.540642
CAACGCA145700.030.617885
AACGCAG151900.029.6126146
ACAACGC7050.029.340763
GTCTCGC11500.028.2088451
TCTGTCG11150.026.9791138
ACGCAGA167950.026.4468737
TACAACG7950.026.0203442
CGCAGAG170250.025.7306848
ATAACGC3500.025.5207463
GTGGTAT29050.024.5997961
TGGTATC29350.024.18782
GTACATG86050.024.0947131
GCAGAGT177700.023.964229
Produced by FastQC (version 0.11.5)