FastQCFastQC Report
Thu 2 Jun 2016
DRR024892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311924
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104240.3147415218465158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.2606340000555568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62150.1876552722828181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67300.048.714451
GTATCAA116000.037.2241481
TACCTGG31600.034.8101922
TATAACG3350.032.2745862
GTACCTG35300.032.246361
TACAACG7700.029.3040872
ATAACGC3850.029.3036463
ATCAACG146800.028.947663
ACAACGC7650.028.8806883
TCAACGC148950.028.620614
TATCAAC149450.028.560622
CAACGCA157250.027.2892885
AACGCAG165900.026.1497426
GTGGTAT35200.025.255691
TGGTATC34250.024.5680162
ACCTGGG46350.023.0222193
ACGCAGA186650.023.014987
CGCAGAG184650.022.8824658
GTACAAC12700.021.4814871
GTACATG88750.021.4647921
Produced by FastQC (version 0.11.5)