FastQCFastQC Report
Thu 2 Jun 2016
DRR024891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8450674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148160.17532329373964728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119810.14177567375099312No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA87400.10342370324544527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152750.046.2300151
GTATCAA260100.041.1764341
ATCAACG340250.031.128253
TCAACGC341450.031.03064
TATCAAC352150.030.2260572
CAACGCA386300.027.42795
AACGCAG397800.026.6445966
ACGCAGA414050.025.2469757
GTACATG389600.024.3521631
CGCAGAG428150.024.3056098
TACATGG392350.022.8991722
GCAGAGT444150.022.7209879
ACATGGG412500.021.083343
GTGGTAT80700.020.2740751
AGAGTAC408100.019.33212510-11
CAGAGTA431300.019.06598710-11
GAGTACA288400.018.714661
GTACTTT217450.018.62992114-15
GAGTACT223700.018.33000612-13
CATGGGG302700.018.215834
Produced by FastQC (version 0.11.5)