FastQCFastQC Report
Thu 2 Jun 2016
DRR024891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8450674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179740.2126930940656331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144650.17116977888390913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95700.11324540504106537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.045.5048451
GTATCAA278150.041.7685471
ATCAACG373350.030.861133
TCAACGC375950.030.6553064
TATCAAC388950.029.7804742
CAACGCA405650.028.4919595
AACGCAG417950.027.6647056
ACGCAGA459600.024.888687
CGCAGAG476150.023.865688
GTACATG392600.023.5899451
GCAGAGT489700.022.7350679
TACATGG395850.022.0054072
GTGGTAT84100.020.8614431
ACATGGG416950.020.294263
AGAGTAC449450.019.98178110-11
CAGAGTA477050.018.82572410-11
AGTACTT258950.018.78356612-13
GTACTTT257800.018.48464814-15
GAGTACT259950.018.45820612-13
GAGTACA293400.017.9872281
Produced by FastQC (version 0.11.5)