FastQCFastQC Report
Thu 2 Jun 2016
DRR024890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4259715
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87660.20578841542215853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76200.1788852071089263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44700.10493659787098432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.036.243781
GTATCAA154550.031.3654021
ATCAACG179000.026.500463
TCAACGC181150.026.2613284
TATCAAC187300.025.35392
CAACGCA187500.025.320915
AACGCAG194800.024.3711686
GTACATG98150.024.1435641
TACATGG96700.023.5817742
CGCAGAG207900.022.4953168
ACGCAGA210050.022.4224917
ACATGGG101650.021.9223063
GAGTACT135500.021.07023212-13
AGAGTAC178900.020.61121410-11
GCAGAGT223550.020.5420329
AGTACTT146550.019.35324512-13
GTACTTT156250.018.1969114-15
TATAACG4500.017.7622362
CAGAGTA225650.017.49705110-11
CATGGGG64050.017.1005974
Produced by FastQC (version 0.11.5)