FastQCFastQC Report
Thu 2 Jun 2016
DRR024890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4259715
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96770.22717482272875061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89150.209286302017858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.12735593813201118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112100.033.4820941
GTATCAA169800.030.746851
ATCAACG200050.025.9421583
TCAACGC201550.025.7690854
TATCAAC207750.025.1390382
CAACGCA207100.025.0785065
AACGCAG214550.024.2515016
GTACATG94500.021.9991441
CGCAGAG236500.021.7991168
ACGCAGA237350.021.760657
GAGTACT154200.021.09016812-13
AGAGTAC200650.020.5878310-11
TACATGG97900.019.9749742
GCAGAGT254800.019.8277059
AGTACTT168000.019.315812-13
TATAACG3900.019.2855032
ACATGGG97350.019.2669323
GTACTTT173600.018.70624514-15
GGACCGT3701.6552804E-1017.784676
ATAACGC4154.7293724E-1116.9909923
Produced by FastQC (version 0.11.5)