FastQCFastQC Report
Thu 2 Jun 2016
DRR024889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812225
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133140.4734329578892158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107950.38385975517606163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74210.2638835797277956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42910.15258380819457903No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37570.13359528487229863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.1083128128083635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69850.051.022471
GTATCAA124950.041.580021
TATAACG2600.036.1672332
TACCTGG30100.034.364882
TACAACG6300.033.5838622
ATCAACG159250.032.238793
TAACGCA3650.032.199694
TCAACGC161450.032.030174
TATCAAC162200.031.7700822
CAACGCA170200.030.6017425
AACGCAG177750.029.3516836
GTACCTG37150.028.4762211
GTGGTAT32050.028.3131331
ACCTGGG36150.027.3115583
ACGCAGA194700.026.6752347
TGGTATC33750.026.1904372
CGCAGAG197250.026.1874128
GTACATG129750.025.6919781
TACATGG131750.024.8380362
ACAACGC9100.024.7990653
Produced by FastQC (version 0.11.5)