FastQCFastQC Report
Thu 2 Jun 2016
DRR024889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812225
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150030.5334921636782264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127170.45220421552329565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97650.3472339517641725No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.14973908559948085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36730.13060832614744552No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.1264479193521144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.051.7518961
GTATCAA139450.039.3919451
TACCTGG26900.036.3490682
TACAACG7350.030.6998422
GTACCTG33950.030.1995051
ATCAACG180500.030.1848133
TATAACG3450.029.9768372
TCAACGC182800.029.8259644
TATCAAC183750.029.7788472
CAACGCA193450.028.2563465
ACAACGC8500.027.6524313
ACCTGGG34050.027.6118243
AACGCAG201050.027.5388776
GTGGTAT36850.026.9294871
ATAACGC3950.026.1823023
TGGTATC37600.025.3799322
ACGCAGA227200.024.1813377
CGCAGAG228300.023.8795558
TAACGCA4650.023.2481084
GTACATG129950.022.5111261
Produced by FastQC (version 0.11.5)