FastQCFastQC Report
Thu 2 Jun 2016
DRR024888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557028
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112880.31734358008989527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95800.2693259653845851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.16890505219525964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77250.047.474891
GTATCAA132350.037.4086721
ATCAACG160600.030.3272633
TCAACGC162700.029.9044044
TATCAAC166950.029.3737662
CAACGCA170150.028.6482835
AACGCAG176950.027.5973876
GTACATG114600.025.8478131
ACGCAGA196500.024.7799367
CGCAGAG199900.024.3349558
TACATGG115750.023.9252262
ACATGGG118150.022.5217293
GCAGAGT210600.022.4290399
GTGGTAT39350.022.3441961
TGGTATC40000.020.9228942
GAGTACT138800.020.8410812-13
AGAGTAC188150.020.6587610-11
TACCTGG27550.020.138272
TATAACG3553.6379788E-1219.8666522
CAGAGTA209200.019.36638510-11
Produced by FastQC (version 0.11.5)