FastQCFastQC Report
Thu 2 Jun 2016
DRR024888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557028
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125940.35405962505777294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112760.31700621979922566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78050.21942475572303619No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85250.046.8960841
GTATCAA144900.036.6144681
ATCAACG184200.028.556713
TCAACGC186200.028.2728394
TATCAAC187850.028.051892
CAACGCA193800.027.0913375
AACGCAG202500.026.020266
GTACATG116450.023.9108071
ACGCAGA224100.023.320897
CGCAGAG224900.022.9659488
TACATGG117400.022.5428072
ACATGGG121050.021.4747453
GCAGAGT236400.021.331849
TACCTGG28550.021.0751912
AGAGTAC206950.020.8812410-11
GAGTACT156250.020.00177612-13
AGTACTT160250.019.3705412-13
TATAACG3951.8189894E-1219.0410372
TAGACCG1751.0778929E-418.8014355
TAGCGCG1250.006415605518.7990599
Produced by FastQC (version 0.11.5)