FastQCFastQC Report
Thu 2 Jun 2016
DRR024887_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024887_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561636
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111140.31204760958166416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93070.26131249796441863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.1575961159422243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70900.051.8595921
GTATCAA125250.040.242561
ATCAACG155450.032.1797643
TCAACGC156250.032.0105064
TATCAAC162700.030.6906072
CAACGCA163700.030.5519945
GTGGTAT32100.029.7344251
AACGCAG169300.029.567936
TGGTATC33050.027.030292
ACGCAGA189900.026.2115787
CGCAGAG192250.025.695598
TATAACG3500.025.5243152
GTACATG105000.023.9122521
GCAGAGT204150.023.760339
TACATGG103350.023.1111622
TACCTGG27850.022.1164112
ACATGGG107650.021.2253173
GAGTACT146550.020.63679312-13
AGAGTAC189600.020.61323410-11
CAGAGTA203850.020.23292210-11
Produced by FastQC (version 0.11.5)