FastQCFastQC Report
Thu 2 Jun 2016
DRR024887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561636
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120800.3391699769431801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106370.29865488780998395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70950.1992062074844257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76500.051.0334971
GTATCAA133350.039.858791
ACAACGC6700.031.572873
ATCAACG173550.030.6076643
TCAACGC174400.030.427264
TATCAAC179300.029.5478632
CAACGCA180550.029.4164495
AACGCAG187250.028.48946
GTGGTAT35900.027.5146181
ACGCAGA211750.024.9028037
TACCTGG27950.024.8921452
CGCAGAG210900.024.8917488
TGGTATC36800.024.7820012
GTACATG106850.023.4194011
GCAGAGT224600.022.9130639
TACATGG104900.022.6307182
TAACGCA4450.022.180674
ACATGGG107000.021.0879213
AGAGTAC210700.020.88862610-11
GTACCTG34700.020.4685061
Produced by FastQC (version 0.11.5)