FastQCFastQC Report
Thu 2 Jun 2016
DRR024886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7727604
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148620.19232351968346204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114380.14801483098771626No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120850.051.9321981
GTATCAA233550.045.270121
ATCAACG300400.034.8792573
TCAACGC305350.034.312284
TATCAAC319100.032.91212
CAACGCA343250.030.5238785
AACGCAG354200.029.5923656
ACGCAGA375850.027.5499277
CGCAGAG387050.026.5580778
GTGGTAT50500.025.5071091
GTACATG370350.025.2097741
GCAGAGT402850.024.8630859
TACATGG358850.024.7074552
ACATGGG382250.022.6516173
TGGTATC58750.021.2049482
AGAGTAC388300.020.11771210-11
CAGAGTA401750.019.77177810-11
CATGGGG248950.019.6173734
GTACTTT203550.019.40564714-15
AGTACTT209900.019.2107112-13
Produced by FastQC (version 0.11.5)