FastQCFastQC Report
Thu 2 Jun 2016
DRR024886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7727604
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164770.21322262372657813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135050.17476309603856513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99400.12862977968332745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126700.053.5000421
GTATCAA239100.043.0855181
ATCAACG316050.032.3513073
TCAACGC320200.031.9409084
TATCAAC336800.030.5816922
CAACGCA348400.029.3420735
AACGCAG362150.028.2669586
GTGGTAT51650.027.8687741
GTACATG375450.025.1581331
ACGCAGA402100.025.1174477
TACATGG366700.024.4344842
CGCAGAG415550.024.2140038
TGGTATC59350.023.3664382
GCAGAGT433500.022.766879
ACATGGG391700.022.1787363
AGAGTAC418050.019.7012210-11
CATGGGG253800.019.2412664
AGTACTT235650.018.94596312-13
CAGAGTA431300.018.9052910-11
GTACTTT228600.018.74910414-15
Produced by FastQC (version 0.11.5)