FastQCFastQC Report
Thu 2 Jun 2016
DRR024885_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024885_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4681575
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146500.31292887543187925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119120.2544442842419485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74930.16005297362532908No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87200.058.224921
GTATCAA170900.042.609951
TCAACGC214150.033.78274
ATCAACG214600.033.7779353
TATCAAC221750.032.6686632
CAACGCA225700.032.0532155
GTGGTAT38600.030.813061
AACGCAG236500.030.6067336
TGGTATC37850.029.311532
ACGCAGA266400.026.976867
CGCAGAG270050.026.420228
GTACATG210550.026.3245051
TACATGG208450.025.5517352
GCAGAGT288250.024.0509139
ACATGGG212900.024.0453263
GAGTACT173900.021.17496912-13
CAGAGTA283250.020.60917310-11
AGAGTAC271950.020.37668810-11
CATGGGG107450.020.3432524
AGTACTT179500.019.5717712-13
Produced by FastQC (version 0.11.5)