FastQCFastQC Report
Thu 2 Jun 2016
DRR024885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4681575
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169990.36310429716494985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144190.3079946385564687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94900.202709558214917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90300.059.4372831
GTATCAA174950.043.5036431
ATCAACG232650.032.5566753
TCAACGC235450.032.139584
TATCAAC237500.031.9717032
CAACGCA243550.031.2443685
AACGCAG254450.030.014526
GTGGTAT37400.027.7959631
ACGCAGA291000.026.0335887
CGCAGAG293050.025.7392168
TGGTATC37500.025.702742
GTACATG204450.025.3315221
TATAACG4000.024.6840342
TACATGG204350.024.5267162
GCAGAGT311000.023.8305059
ACATGGG210900.023.0065063
AGAGTAC293850.021.19903210-11
GAGTACT200600.020.61644412-13
AGTACTT205550.020.49721112-13
ATAACGC6000.020.373693
Produced by FastQC (version 0.11.5)