FastQCFastQC Report
Thu 2 Jun 2016
DRR024884_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024884_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444020
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82310.2389939663532732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72510.21053884704502296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.12171822463284186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57550.058.1600881
GTATCAA109300.040.9886051
ATCAACG134050.032.8933033
TCAACGC135100.032.6023864
TATCAAC140350.031.518192
CAACGCA141450.031.3709665
AACGCAG148900.030.0521726
GTGGTAT25600.029.9321561
TGGTATC24300.028.8246522
CGCAGAG165300.026.6136918
ACGCAGA167600.026.5296697
GTCTCGC9250.025.4108181
GTACATG99550.024.2723731
GCAGAGT178900.023.9599889
TCTGTCG9900.023.7376048
TACATGG97900.023.4326382
ACATGGG97600.022.2998873
GTACCGT1100.003059308321.3653956
GAGTACT131150.020.9099312-13
CAGAGTA179750.020.5259310-11
Produced by FastQC (version 0.11.5)