FastQCFastQC Report
Thu 2 Jun 2016
DRR024884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444020
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90950.2640809286821795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82470.2394585397297344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52630.15281560501971533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.054.331541
GTATCAA103450.042.7487561
ATCAACG136200.032.0719263
TCAACGC137850.031.6742344
TATCAAC141050.031.0033682
CAACGCA143550.030.4165335
TGGTATC23350.029.8039512
GTGGTAT25000.029.1687551
AACGCAG151050.029.0605896
ACAACGC5200.028.0313973
CGCAGAG169400.025.5789768
ACGCAGA171600.025.387987
GCAGAGT181700.023.3042729
GTACATG95000.022.7803521
TATAACG2807.6397555E-1121.8315372
AGAGTAC173650.021.77293210-11
TCTGTCG8850.021.7723988
TACATGG95100.021.4589392
GTCTCGC8450.020.600191
AGTACTT137450.020.56631912-13
Produced by FastQC (version 0.11.5)