FastQCFastQC Report
Thu 2 Jun 2016
DRR024883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854609
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165840.4302381901770063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133200.34556034088022936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84080.21812847943851113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88700.056.7667431
GTATCAA171150.044.3632971
ATCAACG215550.035.0047533
TCAACGC218400.034.5264364
TATCAAC221600.034.2408872
CAACGCA229950.032.7308965
AACGCAG238800.031.6708536
GTGGTAT35700.029.762431
TGGTATC36500.028.4649662
ACGCAGA263350.028.4135327
CGCAGAG264050.028.1413088
GTACATG187150.026.8293131
TACATGG183150.026.4644572
GCAGAGT280100.026.1428499
ACATGGG192200.024.1170143
GAGTACT184850.021.66174112-13
CAGAGTA278250.021.57020610-11
AGAGTAC266050.020.9605710-11
CATGGGG90100.020.609734
CATGGGA87000.020.58764
Produced by FastQC (version 0.11.5)