FastQCFastQC Report
Thu 2 Jun 2016
DRR024883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854609
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183840.4769355335391994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154080.3997292591803734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102730.26651211575545014No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40060.10392753194941433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90150.059.0200161
GTATCAA174400.043.833821
ATCAACG226650.033.4389343
TCAACGC228900.033.038764
TATCAAC236050.032.2870032
CAACGCA238850.031.8001825
GTGGTAT36300.031.4921341
AACGCAG248600.030.570716
TGGTATC35650.030.3331182
ACGCAGA280550.026.7859237
CGCAGAG281250.026.668788
GTACATG186550.025.64651
TACATGG182350.024.9327092
GCAGAGT296900.024.6615319
ACATGGG190700.022.9531383
GAGTACT194650.021.05352612-13
AGAGTAC279900.021.0299210-11
CAGAGTA292900.020.87472310-11
AGTACTT196200.020.56383512-13
GTACTTT200650.020.0960614-15
Produced by FastQC (version 0.11.5)