FastQCFastQC Report
Thu 2 Jun 2016
DRR024882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205069
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100210.31266097547353894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85050.26536090174657706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52090.16252380213967313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58150.054.886631
GTATCAA106650.041.473941
TCAACGC133600.032.5779654
ATCAACG133550.032.5549663
TATCAAC140400.031.1679882
CAACGCA139800.031.166785
AACGCAG145150.030.0175556
GTGGTAT24650.027.4594651
ACGCAGA162950.026.5065677
CGCAGAG163200.026.2927578
TACCTGG29400.025.4200252
GTACATG98400.025.1745681
GTACCTG31750.024.2798861
TGGTATC27550.024.0560172
GCAGAGT173000.023.9340049
TACATGG97700.023.6699182
ACATGGG99800.021.9469813
AGAGTAC165050.020.5877210-11
CAGAGTA171900.020.46451410-11
GTACACG4150.020.3878251
Produced by FastQC (version 0.11.5)