FastQCFastQC Report
Thu 2 Jun 2016
DRR024882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205069
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110760.34557758350912254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94390.2945022400453781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.19671963380507562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56600.057.2600561
GTATCAA106450.040.255171
ATCAACG136500.030.8608153
TCAACGC139300.030.1982634
TATCAAC142550.029.5844862
CAACGCA145350.029.1676565
AACGCAG151700.027.945866
GTGGTAT25500.026.5626411
ACAACGC6700.025.9632453
GTACATG98050.025.1381131
TACCTGG29350.024.9893682
GTACCTG31500.024.6389581
ACGCAGA171350.024.437857
TGGTATC26350.024.2659362
TACATGG96500.023.9217512
CGCAGAG175150.023.8271588
TATAACG3250.023.1459712
GCAGAGT185950.022.1147129
GTACCGG3900.021.709851
ACATGGG100850.021.5842573
Produced by FastQC (version 0.11.5)