FastQCFastQC Report
Thu 2 Jun 2016
DRR024881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015931
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83610.27722782782497346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67640.22427568800479852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42180.1398573110591721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.055.7722241
GTATCAA94750.044.6018031
ATCAACG118000.035.0949173
TCAACGC118900.034.8688054
TATCAAC122150.033.9416282
CAACGCA123750.033.4262585
AACGCAG129700.032.1800966
GTGGTAT21350.029.9442161
ACGCAGA143100.028.8706367
CGCAGAG144100.028.571968
TGGTATC22600.026.2071422
GCAGAGT157750.025.2654139
GTACATG79500.022.6466311
TCTGTCG8900.022.179888
AGAGTAC147100.021.77474610-11
CTGTCGC9950.021.7287489
TACCTGG21800.021.346972
TACATGG80150.021.2306162
GTCTCGC8900.021.12721
AGTACTT119100.020.50025712-13
Produced by FastQC (version 0.11.5)