FastQCFastQC Report
Thu 2 Jun 2016
DRR024881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015931
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.31204294793216425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82350.27305001341211055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.17974549152483926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49600.057.2859841
GTATCAA97750.041.6286621
ATCAACG123900.032.599133
TCAACGC126300.031.8542424
TATCAAC129350.031.3356952
CAACGCA130850.030.674755
AACGCAG137500.029.395816
TGGTATC21600.028.0824172
GTGGTAT22050.027.7349821
ACGCAGA154050.026.0838957
CGCAGAG154950.025.871738
ACAACGC5000.025.3908863
GTACATG78900.023.6704521
GCAGAGT167000.023.4139639
TACATGG79000.022.4394782
TACCTGG23450.022.2576182
AGAGTAC157900.021.26611510-11
ACATGGG80200.020.9304223
GTACCTG24300.020.7143151
CAGAGTA164750.019.89696710-11
Produced by FastQC (version 0.11.5)