FastQCFastQC Report
Thu 2 Jun 2016
DRR024880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5421136
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129270.23845555617863118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108050.19931246882572215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67300.12414372190625729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86850.055.5945051
GTATCAA172100.043.2991751
ATCAACG213750.034.1542243
TCAACGC216800.033.6517454
TATCAAC226000.032.4292452
CAACGCA229050.031.8519885
AACGCAG238600.030.6519576
GTGGTAT41450.027.5621431
ACGCAGA265450.027.1432867
CGCAGAG265550.027.0440718
TGGTATC41750.026.5738622
GTACATG202650.025.9837991
TACATGG197350.025.27422
GCAGAGT284300.024.4832579
ACATGGG206600.023.140343
AGAGTAC269800.020.42499210-11
GAGTACT176850.020.40915912-13
CAGAGTA281900.020.356910-11
AGTACTT180650.019.75871812-13
GTACTTT187000.019.15033114-15
Produced by FastQC (version 0.11.5)