FastQCFastQC Report
Thu 2 Jun 2016
DRR024880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5421136
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149640.2760307064792324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123950.22864211486301025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81220.14982099692758122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89500.054.975371
GTATCAA175050.043.290531
ATCAACG225300.033.097343
TCAACGC229200.032.6053854
TATCAAC237200.031.7350882
CAACGCA240350.031.171025
AACGCAG251250.029.89196
GTGGTAT41800.027.5707951
ACGCAGA282700.026.2161337
TGGTATC41700.026.158632
CGCAGAG284450.025.9722358
GTACATG202050.025.9582561
TATAACG4800.025.4680212
TACATGG196400.025.1368522
GCAGAGT303350.023.9047749
ACATGGG205400.023.3022793
AGAGTAC287450.021.26232710-11
AGTACTT199550.020.1361812-13
GAGTACT193100.019.72574812-13
CAGAGTA300650.019.55504610-11
Produced by FastQC (version 0.11.5)