FastQCFastQC Report
Thu 2 Jun 2016
DRR024879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354349
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173330.5167321587586742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137460.4097963569085984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88680.2643732062465772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37660.11227215772717745No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.11167591684705437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84350.054.2308581
GTATCAA160050.042.1810651
TATAACG3050.040.0719452
TACCTGG27250.035.190922
GTGGTAT35650.032.7047541
ATCAACG210300.031.8738293
TCAACGC212850.031.5577534
TATCAAC216600.031.0778882
CAACGCA224800.029.9211245
TGGTATC38500.029.6696552
GTACATG198100.029.498871
ACCTGGG33800.029.0658873
AACGCAG236300.028.6206726
TACATGG195300.028.618482
ACATGGG200450.026.8036633
GTACCTG35900.026.7149561
GTACCGG3100.025.781371
CGCAGAG260800.025.5892228
ACGCAGA263050.025.4954077
GCAGAGT272400.023.8093919
Produced by FastQC (version 0.11.5)