FastQCFastQC Report
Thu 2 Jun 2016
DRR024879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354349
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193140.575789817934866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158810.47344507086173804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107420.32024097671411056No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.1267608111141685No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.10192737845704189No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34080.1015994459729742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86050.056.5793531
GTATCAA163600.043.3309751
TACCTGG26000.034.9021072
TATAACG4300.032.8035242
ATCAACG215350.032.7492333
TCAACGC217950.032.3038444
TATCAAC223600.031.5839022
GTGGTAT34350.031.3600851
TGGTATC33800.031.1601092
CAACGCA227750.031.1408275
AACGCAG241000.029.6804896
GTACCTG33400.028.0269031
GTACATG186000.027.010141
TACATGG183050.026.4052962
ACCTGGG32400.026.2656573
TAGCGCG909.521313E-426.1111984
ACAACGC9400.026.0093923
ACGCAGA272600.025.9636677
CGCAGAG270950.025.86168
TAACGCA6550.024.3970284
Produced by FastQC (version 0.11.5)