FastQCFastQC Report
Thu 2 Jun 2016
DRR024878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234796
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64930.29054106057107676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.2456152597373541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.14484543555653404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.046.5760771
GTATCAA75750.037.048951
GTCTCGC7100.033.105171
TCTGTCG7000.031.5577688
ATCAACG89900.031.2147583
TCAACGC90300.031.0244314
CAACGCA94600.029.6142315
TATCAAC94750.029.4687862
TACCTGG19800.029.4382212
GTACCTG21250.029.4223181
ACAACGC4700.029.0031663
AACGCAG98200.028.7659786
TCTCGCT8000.027.6161352
TAACGCA2450.026.8600794
TACAACG5100.026.7290082
ACGCAGA108600.025.7076877
CGCAGAG110300.025.2688588
GTACATG54950.025.1514951
ATAACGC2151.879016E-924.0491393
TATAACG2202.4501787E-923.5030942
Produced by FastQC (version 0.11.5)