FastQCFastQC Report
Thu 2 Jun 2016
DRR024878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234796
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74120.3316633822505499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66200.29622390589566117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42920.1920533238828063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.050.6746031
GTATCAA84150.037.50461
ATCAACG103050.030.1111533
TCAACGC104150.029.8289224
TACCTGG18150.029.5297432
CAACGCA108400.028.8762195
TATCAAC107950.028.8750232
AACGCAG111500.028.199216
GTACCTG21000.025.5329931
ACGCAGA124000.025.1280087
CGCAGAG125000.024.9269858
ACAACGC3850.024.4230963
GTCTCGC6650.024.0476951
GCAGAGT131600.023.284029
GAGTACT95450.022.05811112-13
TACACGC3650.021.8902845
GTACATG51750.021.7221971
AGAGTAC117250.021.36390110-11
TCTCGCT7750.020.6256982
CAGAGTA130800.020.56998410-11
Produced by FastQC (version 0.11.5)