FastQCFastQC Report
Thu 2 Jun 2016
DRR024877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429226
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81010.23623406564630034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75150.21914566144080327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40470.11801496897550642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.053.070821
GTATCAA110750.040.7102971
ATCAACG134100.033.022773
TCAACGC134500.032.9939844
TATCAAC138500.031.9415932
CAACGCA141050.031.4284975
AACGCAG147250.030.1650856
GTGGTAT27850.029.542161
TGGTATC28900.027.3292772
CGCAGAG161700.027.1202288
ACGCAGA162400.027.0612167
GCAGAGT174100.024.675329
GTACATG92200.023.201171
TACATGG91750.021.8795572
GAGTACT124700.021.67114812-13
ACATGGG91050.021.0138593
CAGAGTA174350.020.91968510-11
AGAGTAC162900.020.7310410-11
TCTGTCG9400.020.5011258
TATAACG2401.5775913E-719.5886942
Produced by FastQC (version 0.11.5)