FastQCFastQC Report
Thu 2 Jun 2016
DRR024877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429226
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87340.2546930415201564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82400.24028745845272376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.14175210382751094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62400.053.375861
GTATCAA109750.038.8346941
ATCAACG138250.030.50763
TCAACGC139100.030.3105664
CAACGCA145400.029.1265535
TATCAAC146500.028.9500712
AACGCAG151850.028.0111436
GTGGTAT28450.027.4487151
TGGTATC28300.025.7528932
GTACATG92950.025.2043881
CGCAGAG168200.024.839048
ACGCAGA169300.024.8164487
TACATGG92800.023.712572
GCAGAGT179000.022.9465549
ACATGGG94950.022.0366613
CTATGCG1554.2445565E-521.223919
AGAGTAC168800.021.18708810-11
TATAACG3600.020.8976882
GTCTCGC9200.019.9421981
CAGAGTA180500.019.85279710-11
Produced by FastQC (version 0.11.5)