FastQCFastQC Report
Thu 2 Jun 2016
DRR024876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352499
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186380.5559434917057394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145220.433169405867086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99290.2961671278649151No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42450.12662196170677456No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36960.11024611789593375No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35510.10592098610618526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90050.057.9951971
TACCTGG26450.045.4935722
GTATCAA179700.044.260261
TATAACG4400.042.7310032
TACAACG6500.036.8801422
ACCTGGG33650.034.6419563
GTACCTG35850.034.6159671
ATCAACG228900.034.395753
TCAACGC232150.033.873734
TATCAAC242250.032.6748852
CAACGCA243650.032.313035
TAACGCA6150.032.100364
AACGCAG256400.030.8693476
GTACCGG2200.029.9134861
GTACCCG2450.028.779741
TGGTATC36500.028.4600182
GTGGTAT37300.028.229351
ACGCAGA278500.028.0649287
CGCAGAG277700.027.942688
GTACATG203200.026.5107861
Produced by FastQC (version 0.11.5)