FastQCFastQC Report
Thu 2 Jun 2016
DRR024876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352499
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212360.6334379219799916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172700.5151381104065952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124570.3715735634820473No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44060.13142434941815045No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39920.11907535244604099No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39310.11725581424483647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97650.055.7854581
TATAACG4050.041.7937932
GTATCAA184350.041.670411
TACCTGG30400.039.4394572
ACCTGGG32850.035.495043
TACAACG8700.031.885782
GTACCTG38650.031.7669641
ATCAACG242200.031.5450063
TCAACGC246250.031.0146244
ACAACGC9250.030.4972633
TATCAAC254350.030.0575182
GTGGTAT39200.029.8811951
CAACGCA257150.029.827925
TGGTATC39450.029.080852
AACGCAG270750.028.4672376
GTACATG202050.027.263571
TAACGCA6100.026.9666864
TACATGG196050.026.8126182
ACGCAGA300050.025.4677377
CGCAGAG299500.025.4203598
Produced by FastQC (version 0.11.5)